Present study was designed to evaluate the cardioprotective effect of acute administration of lycopene on isoproterenol (ISO)-induced myocardial infarction in rats. Lead II electrocardiograph was monitored and recorded at regular intervals. Cardiac marker enzymes such as serum glutamate oxaloacetate transaminase (SGOT), creatine kinase (CK) and lactate dehydrogenase (LDH) were estimated in serum sample whereas level of lipid peroxidation and biomarkers of oxidative stress in heart tissues were measured. ISO (200mg/kg, sc twice at the interval of 24 hours) induced pathological changes in rat heart as evident by a significant increase in the levels of SGOT, CK, LDH and lipid peroxidation and a significant decrease in the activities of superoxide dismutase, catalase and reduced glutathione in heart. In addition the ECG exhibited significant reduction in R amplitude and increase in ST-segment elongation and ST-segment elevation in ISO treated rat indicating severe myocardial ischemic injury. Finally, blinded histopathological studies confirmed necrosis and inflammatory cells in isoproterenol-treated rats. Administration of lycopene (1, 3 and 6mg/kg, i.p) 10min before isoproterenol treatment significantly attenuated isoproterenol induced cardiac dysfunction, myocardial injury and histological alteration in dose dependent manner. The results of present study suggest that lycopene has a significant protective effect against isoproterenol-induced myocardial infarction after acute administration which may be accounted for its potent antioxidant and protective property.
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